Separation of HDL<sub>2</sub> and HDL<sub>3</sub> from Human Serum by Zonal Ultracentrifugation
نویسندگان
چکیده
منابع مشابه
Isolation of human serum HDL, by zonal ultracentrifugation
High density lipoprotein subfraction-1 (HDLI) is thought to interact with the high-affinity apoprotein B, E receptors of peripheral cells and may act as a modulator of LDL binding and uptake. In the present study the concentration and composition of HDLl in normal and hypercholesterolemic sera were studied using zonal ultracentrifugation. To permit separation of the HDLl from VLDL, LDL, and Lp(...
متن کاملIsolation of human serum HDL1 by zonal ultracentrifugation.
High density lipoprotein subfraction-1 (HDL(1)) is thought to interact with the high-affinity apoprotein B, E receptors of peripheral cells and may act as a modulator of LDL binding and uptake. In the present study the concentration and composition of HDL(1) in normal and hypercholesterolemic sera were studied using zonal ultracentrifugation. To permit separation of the HDL(1) from VLDL, LDL, a...
متن کاملSeparation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation.
The major lipoprotein density classes (chylomicrons-VLDL, LDL, HDL(2) and HDL(3)) were isolated from human plasma in a two-step ultracentrifugal procedure using the Ti-14 zonal rotor. The isolation of the two major high density lipoprotein subclasses (HDL(2) and HDL(3)) was achieved in a 24-hr run using a nonlinear NaBr gradient in the density range of 1.00-1.40. The lipoproteins with a density...
متن کاملThe isolation of lipoproteins from human plasma by ultracentrifugation in zonal rotors.
The major classes of lipoproteins were isolated from human plasma by ultracentrifugation in continuous density gradients using the Ti-14 and Ti-15 zonal rotors. Chylomicrons + VLDL, LDL, and HDL were separated from each other and from the more dense residual proteins (albumin fraction) of plasma by rate-zonal flotation in NaBr gradients in the density range 1.0-1.4. The chylomicron-VLDL fractio...
متن کاملSeparation of the principal HDL subclasses by iodixanol ultracentrifugation.
HDL subclasses detection, in cardiovascular risk, has been limited due to the time-consuming nature of current techniques. We have developed a time-saving and reliable separation of the principal HDL subclasses employing iodixanol density gradient ultracentrifugation (IxDGUC) combined with digital photography. HDL subclasses were separated in 2.5 h from prestained plasma on a three-step iodixan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1982
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.9.6_933